Ligation Calculator
The ligation calculator can be used to calculate the optimal vector, and insert mass for a cloning reaction.
Do you still not know these terms and topics? The article below will cover all of the basics. We will also discuss vector ligation reactions, and how to use the cloning ratio calculator as well as the vector ligation formula .
Our vector insert calculator works in both directions. Simply input all data and you will get your results.
How do I use the ligation calculator
You’ve found the best neb-ligation calculator. Just a few steps away is your solution.
- Enter your length in either base pairs or kilobases.
- Your vector length can be entered in either base pairs or kilobases.
It is easy to convert these values:
1000 bp = 1 Kb
1 bp = 0.01 kb
- Enter your vector Mass in nanograms or micrograms.
- Select the insert/vector.
The ideal ratio should be 3:1. The ideal ratio is 3:1.
- In this case, the insert required for the reaction will be your result.
We recommend that you add at least 50 ng.
How do you calculate the molar ratio
The following equation is used to calculate the ligation molar rate calculator:
Require insert mass = (Vector weight * Insert length/Vector length * Molar rate
where:
- The required insert mass is listed in nanograms .
- Nanograms (ng), are the units of vector mass.
- Kilobases (kb),, insert length
- kilobases (kb), are used to indicate the vector length.
- The Molar ratio for vector/ is 1.
Our molar relationship calculator can be used to calculate the molar percentage of a balanced chemical reactions.
Ligation reaction
The ligation is intended to create a -recombinant plasmid. To do this, we will need to covalently link two sticky ends: one from the DNA backbone and one from the DNA insert. The enzyme – T4 DNA ligase – ATP is used to generate energy.
What sticky ends are?
Most restriction ( scissor), enzymes slice the DNA asymmetrically. This results in unbalanced ends. Later, we might join them to create compatible tips.
What’s a vector?
A vector biological molecule acts as a carrier for a specific gene or expression (e.g. plasmids). It is used to implant desired genetic material in a foreign cell.
Mathematical Vectors have a different definition of vectors in biology.
Vector = Backbone + Insert
The backbone , which is the longer sequence that supports the vector, is called .
On what does the insert/vector ratio depend?
The molar ratio is determined by the size of each ingredient and their content . A ratio 3 is recommended in most cases.
Why do we need ligation?
- In the molecular Cloning of DNA, we use ligation. Our desired DNA fragment is multiplied by bacteria that has been implanted with plasmids.
- It can be used for substance production (e.g. insulin);
- They are used as models for diseases.
- They are used in gene therapy.
- Ligation is an important part of DNA replication
- Ligation keeps us alive.
- Enzymes repair the DNA strain through ligation if it is broken. This protects us from unwanted mutations as well as their effects on our bodies.